Comparison of the A-T rich regions and the Bacillus subtilis RNA polymerase binding sites in phage ?29 DNA

摘要

By using a modification of the BAC spreading method for mounting the DNA for electron microscopy, partial denaturation maps of protein-free ?29 DNA and of ?29 DNA containing protein p3 were obtained. In ?29 P3-DNA1 the protein does not seem to influence the melting of the ends of the molecules. The comparison of the partial denaturation map and the B. subtilis RNA polymerase binding sites indicates that five of the seven early promoters (Al, A2, A3, B2 and C2) are located in A-T rich DNA regions whereas the other two early promoters (Bl and Cl) are located in less A-T rich sites.