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29 PHAGE 29 DNA POLYMERASE CHIMERA

机译:29 PHAGE 29 DNA聚合酶

摘要

The present invention belongs to the field of biotechnology. Specifically, the invention includes a carboxy-terminal (C-terminal) region comprising an amino terminal (N-terminal) region encoding a Ph29 type DNA polymerase and at least one HhH domain linked through a linked amino acid sequence To DNA polymerase chimeras and to their use for replicating, amplifying or sequencing template DNA. Similarly, the present invention provides a method for replicating, amplifying or sequencing deoxyribonucleic acid using the DNA polymerase chimera and a kit for carrying out the method.
机译:本发明属于生物技术领域。具体地,本发明包括羧基末端(C末端)区域,其包含编码Ph29型DNA聚合酶的氨基末端(N末端)区域和通过连接的氨基酸序列与DNA聚合酶嵌合体和它们用于复制,扩增或测序模板DNA的用途。类似地,本发明提供了使用DNA聚合酶嵌合体复制,扩增或测序脱氧核糖核酸的方法以及用于实施该方法的试剂盒。

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