Site-specific serine recombinases from Streptococcus pyogenes phage SF370.1 and Bacillus subtilis pahge recombinase SPbetac2and methods of their use

摘要

A method for obtaining site-specific recombination in an isolated eukaryotic cell comprising: - Providing an isolated eukaryotic cell that comprises a first recombination site and a second recombination site; - Contacting the first and second recombination site with a prokaryotic recombinase polypeptide resulting in recombination between the two sites; -The first recombination site is either a phage genomic recombination attachment site (attP) or a bacterial genomic recombination attachment site (attB) and the second recombination is either attP or attB, provided that when the first recombination site is attP the second recombination site is attB or vice versa; - The recombinase is a Streptococcus pyogenes phage recombinase or a Bacillus subtilis phage recombinase, in particular, the recombinases are SF370.1 or SPbetac2 recombinase. The method also encompasses the use of pseudo attP and attB recombination sites as well as a third and fourth, and a fifth and sixth recombination site.