Determination of Picogram Levels of Diacerein in a Pharmaceutical Formulation by Flow-Injection Chemiluminescence

摘要

A simple, sensitive and inexpensive method for determination of diacerein by flow-injection chemiluminescence was proposed, based on the quenching effect of diacerein on the luminol-protein (bovine serum albumin, BSA) reaction. It was found that the decrement of CL intensity was linearly proportional to the logarithm of diacerein concentration ranging from 5.0 to 7.0×103 pg·mL-1 (r = 0.9968), with the limit of detection (LOD) of 1.0 pg·mL-1 (3σ). The proposed procedure was successfully applied to the determination of diacerein in pharmaceutical formulation, human saliva, and serum samples without interference from its potential impurities, with the recoveries ranging from 96.4% to 104.0% and the relative standard deviations (RSDs) less than 4.0% (n=6).