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Determination of Picogram Levels of Roxithromycin in Pharmaceutical, Human Serum, and Urine by Flow-Injection Chemiluminescence

机译:流动注射化学发光法测定药物,人血清和尿液中罗红霉素的皮克水平

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A sensitive chemiluminescence (CL) method, based on the inhibitory effect of roxithromycin (ROX) on the CL reaction between luminol and dissolved oxygen in a flow-injection system, was first proposed for the determination of ROX at picogram levels. The decrement of CL intensity was linearly proportional to the logarithm of ROX concentrations ranging from 0.1 to 100 pg mL−1, giving the limit of detection (LOD) of 0.03 pg mL−1(3σ). At a flow rate of 2.0 mL min−1, a complete analytical procedure including sampling and washing could be performed within 0.5 min, with relative standard deviations (RSDs) of less than 5.0% (n=5). The proposed procedure was applied successfully to the determination of ROX in pharmaceutical, human serum, and urine with the recoveries ranging from 90.0 to 110.0%.
机译:基于罗红霉素(ROX)对流动注射系统中鲁米诺和溶解氧之间的CL反应的抑制作用,提出了一种灵敏的化学发光(CL)方法,用于在皮克级测定ROX。 CL强度的降低与ROX浓度从0.1到100µpg·mL-1的对数成线性比例,检测限(LOD)为0.03µpg·mL-1(3σ)。在2.0?mL?min-1的流速下,可以在0.5分钟内执行包括采样和清洗的完整分析程序,相对标准偏差(RSD)小于5.0%(n = 5)。该方法成功用于药物,人血清和尿液中ROX的测定,回收率从90.0%到110.0%。

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