Diagnostic performance of RT-qPCR method by targeting 85B mRNA in the laboratory diagnosis of Mycobacterium tuberculosis infection: A preliminary study in Turkish patients

摘要

Background: The problems with any PCR method using DNA sequences as targets for amplification include false-positive results, the inability to detect a difference between viable and nonviable organisms and the inability to determine drug susceptibility. Instead of DNA detecting assays, using RT-qPCR to assess the expression of the 85B mRNA gene of M. tuberculosis indicates the presence of a viable organism. In this study, we used the mRNA coding for 85B antigen complex which is present in all mycobacteria.