In vitro antioxidant and H+, K+-ATPase inhibition activities of Acalypha wilkesiana foliage extract

摘要

Aims:The aim of this study was to evaluate the antioxidant activty and anti-acid property of Acalypha wilkesiana foliage extract.Materials and Methods:Hot and cold aqueous extracts were prepared from healthy leaves of A. wilkesiana. Free radical scavenging activity and H+, K+-ATPase inhibition activities of aqueous foliage extracts was screened by in vitro models.Statistical Analysis Used:All experiments were performed in triplicate and results are expressed as mean ± SEM.Results:A. wilkesiana hot aqueous extract (AWHE) showed significant antioxidants and free radical scavenging activity. Further, AWHE has shown a potent H+, K+-ATPase inhibitory activity (IC50: 51.5 ± 0.28 μg/ml) when compare to standard proton pump inhibitor omeprazole (56.2 ± 0.64 μg/ml); however, latter activity is equal to A. wilkesiana cold aqueous extract (AWCE). Quantitative analysis of AWHE has revealed more content of phenols and flavonoids; this is found to be the reason for good antioxidant activity over AWCE. Molecular docking was carried out against H+, K+-ATPase enzyme crystal structure to validate the anti-acid activity of A. wilkesiana major phytochemicals.Conclusions:The present study indicates that the constituents of AWHE and AWCE have good antacid and free radical scavenging activity.