Antioxidant Activity of Crude Bromelain of Pineapple (Ananas comosus (L.) Merr) Crown from Subang District, Indonesia

摘要

Background:Antioxidants are chemical compounds that can donate one or more electrons to free radicals to preventdegenerative diseases. The crown of pineapple (Ananas comosus (L.) Merr.) contains bromelain, that is, aproteolytic enzyme, which can act as an antioxidant.Aims and Objectives:The aim of this study was to determine the antioxidant activity of crude bromelain from pineapple crowns thatwere collected from Subang district, West Java, Indonesia.Materials and Methods:Antioxidant activity was determined by the method of 2,2-diphenyl-1-picrylhydrazyl(DPPH) with vitamin C as astandard and measured with visible spectrophotometer.Results:The pineapple crown was produced 0.26% of dried crude bromelain with total protein content 44.10% and ICvalue 3624 μg/mL of crude bromelain, which was equivalent to 1590.18 μg/mL of total protein.Conclusion:Crude bromelain has medium antioxidant activity.