Genotype and Allele Frequencies of MDR-1 C3435T Polymorphism in Turkish Population

摘要

The response to the medications and the adverse effects of the drugs varies between individuals in the same population due to genetic variation in drug metabolizing enzymes. Single nucleotide polymorphism at 26 exon (C3435T) of MDR1 gene decreases the expression of P-glycoprotein and cause to variability of expression between individuals. Furthermore, interethnic difference of MDR1 gene polymorphism is also present. One hundred seventy four healthy subjects showing a homogeneous distribution through the country, without any remarkable medical or family history were investigated. Polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) assay was applied to assess C3435T SNP MDR1 polymorphism. The mean age of 174 subjects was 35.1 years (±14.74), varying between 10-86 years. 56.3 % (n=98) of the subjects were male and 43.7 % (n=76) were female. Allele distribution was 51 % for C allele and 49 % for T allele. Genotype distribution was 25.8 % for TT, 28.2 % for CC and 46 % for CT. Totally 72 % of the population carries the defective allele. There was no relation between sexes and genotype. This study shows the distribution of allelic variation of MDR1 gene in Turkish population. The data obtained may be useful in clinical pharmacogenetic investigations and epidemiological studies of the MDR1 gene variation.