Chromosomes of a balanced translocation case evaluated with atomic force microscopy

摘要

A couple was referred to our genetics department for cytogenetic analysis because of two previous abortions. The cytogenetic analysis of the male was found as 46, XY and the female revealed a balanced translocation; 46, XX,t (7;12) (p21;q14) and also she had 14 cenh+ as her mother. Atomic force microscopy (AFM) is a useful method for detecting detailed structures of chromosomes. With the help of this new technique the surface topography of human chromosomes can be examined. We used AFM in order to analyse the surface topography of derivative chromosomes of the patients, and found a 0.6 |ìm gap region. In this study, we aimed to examine the differences between the images of the derivative chromosomes detected by light and atomic force microscopy analyses.