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Circular transcriptome sequencing of the middle silk gland and posterior silk gland in the Bombyx mori

机译:家蚕中部丝腺和后丝腺的环形转录组测序

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Circular transcriptome sequencing of the middle silk gland (MSG) and posterior silk gland (PSG) in the Bombyx mori are presented. The middle silk gland and posterior silk gland were collected from the third day of fifth-instar B.mori larvae. The circular RNAs enriched by using RNase R to degrade the linear RNA molecules, and circular RNA sequencing (circRNA-seq) was performed using an Illumina Hiseq. 2500 sequencing platform. Samples are described in the SRA portal (SRP100385) and FASTQ files have been deposited in Sequence Read Archive (accession numbers: SRX2577343 and SRX2577342). The interpretation of these data is presented in the following research article: “Identification of circular RNA in the Bombyx mori silk gland” [] (Gan et al., 2017).
机译:介绍了家蚕中丝腺(MSG)和后丝腺(PSG)的转录组测序。从五龄幼虫B.mori幼虫的第三天开始收集中丝腺和后丝腺。通过使用RNase R降解线性RNA分子富集了环状RNA,并使用Illumina Hiseq进行了环状RNA测序(circRNA-seq)。 2500测序平台。在SRA门户网站(SRP100385)中描述了样本,FASTQ文件已存储在Sequence Read Archive(登录号:SRX2577343和SRX2577342)中。以下研究文章介绍了这些数据:“家蚕丝腺中环状RNA的鉴定” [](Gan等人,2017)。

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