DNA Barcoding in a Crop Genebank: The Capsicum annuum Species Complex

摘要

Variability within eight cpDNA introns including trnS-trnfM, trnL-trnT, trnH-psbA, trnF-trnL, trnD-trnT, trnCrpoB,rps16 and matK, and the nuclear waxy introns was examined in seven species of Capsicum (C. annuum, C.baccatum, C. chinense, C. frutescens, C. pubescens, C. chacoense and C. rhomboideum) in order to evaluate thefeasibility of utilizing these loci for DNA barcoding within the C. annuum complex. Numerous insertions/deletions(indels) and substitutions were detected in all cpDNA introns. However, none was sufficient to differentiate the individualmembers of the C. annuum complex (C. annuum, C. chinense and C. frutescens). Variation within trnL-trnT, trnF-trnLand trnH-psbA enabled the differentiation of the complex from the other taxa examined. In contrast, single base indels andsubstitutions within the waxy introns permitted the differentiation of all taxa within the plant materials examined. The useof trnH-psbA or trnL-trnT, and the waxy introns is proposed for barcoding members of the C. annuum complex.