Potentiometric Method for Evaluating the Oxidant/Antioxidant Activity ofSeminal and Follicular Fluids and Clinical Significance of this Parameterfor Human Reproductive Function

摘要

Potentiometric method based on the sharp change of electrode potential for the ferricyanide/ferrocyanidemediator system after addition of the seminal or follicular fluid sample into the measuring electrochemical cell has beendeveloped to enable the easy assessment of the oxidant/antioxidant status of these kind of biological fluids. Platinumscreen printed electrode has been used as an indicator. Essential advantage of the proposed method is simultaneous use ofthe both forms of the ferricyanide/ferrocyanide mediator system (oxidized and reduced ones) that allows estimatingoxidant and antioxidant components of the seminal or follicular fluids samples which were obtained, respectively, from 99male and 27 female subjects. Oxidant activity of seminal fluids has been observed in pathology conditions of reproductivesystem of male studied subjects and related with possible male infertility symptoms.