Markers of oxidative stress and antioxidant status in the plasma, urine and saliva of healthy mice.

摘要

Oxidative stress markers are usually measured in plasma,a stable environment for biomarkers. Blood collection is invasive,but the use of alternative biofluids is limited, due to highvariability. In this study, we aimed to establish reference valuesfor oxidative stress markers in plasma, urine and saliva of adult,healthy mice and to identify some sources of variability. Sampleswere obtained from 41 female and 37 male adult, healthy mice ofthe CD-1 strain, aged 95-480 days, weighing 21-55 grams.Reference ranges of TBARS (thiobarbituric acid reactivesubstances), AOPP (advanced oxidation protein products),fructosamine, GSH/GSSG (reduced and oxidized glutathione)ratio, TAC (total antioxidant capacity), and FRAP (ferric reducingantioxidant power) were measured in plasma and urine, andTBARS, GSH/GSSG ratio, TAC and FRAP in saliva, using standardspectrophotometric and fluorometric methods. SalivaryGSH/GSSG and urinary AOPP were higher in females. Urinaryfructosamine, GSH/GSSG and FRAP were higher in males. UrinaryTAC and FRAP negatively correlated with age, and urinaryGSH/GSSG positively correlated with weight. We determined thaturine and saliva can be obtained non-invasively from mice, insufficient amounts for reliable oxidative status assessment.Further studies are needed to uncover whether these biofluidsreflect systemic oxidative status in diseases.