The effects of psychological and oxidative stress on the temporal profile of stress proteins and antioxidant status during atherogenesis in Japanese quail.

摘要

This thesis reports the results of four experimental studies which were designed to examine the effects of psychological stress on the interrelationships of heat shock proteins (HSP) and endogenous antioxidants during atherogenesis in an avian model, the atherosclerosis-susceptible (SUS) Japanese quail.; The first study examined the effect of psychological stress in quail fed a cholesterol-supplemented diet for four weeks. As expected, cholesterol-supplemented birds showed significant increases in plaque scores compared to birds fed a non-cholesterol-supplemented diet. The heterophil/lymphocyte ratio was increased in birds exposed to stress and in cholesterol-supplemented birds. Stress or cholesterol feeding alone increased HSP 70 in aortic and heart tissues, whereas lower HSP 70 levels were detected in cholesterol fed birds exposed to stress compared to non-stressed cholesterol fed birds. The stress protocol lowered aortic catalase and heart superoxide dismutase levels, and had little effect on enzymes of the glutathione (GSH) redox cycle. A functional decrease in the antioxidant capacity of the heart as measured by thiobarbituric-acid reactive substances (TBARS) was detected following stress alone or cholesterol supplementation.; In the next longer-term study, I examined the effects of temporal stress during atherogenesis. There were no differences in plaque score between early stressed, recently stressed and non-stressed cholesterol-supplemented birds. Moreover, cholesterol-fed birds exposed to early stress had lower plasma cholesterol levels than cholesterol-fed birds exposed to recent stress or no stress, suggesting that stress may have preconditioned the birds during cholesterol feeding. In general, stressed cholesterol-fed birds had lower HSP 70 and heme oxygenase (HO-1) levels in their aortic and heart tissue compared to non-stressed cholesterol-fed birds. Furthermore, cholesterol-supplementation alone also increased heart and aortic ferritin and catalytic iron levels, and increased generation of TBARS which is indicative of increased oxidative stress.; In the third study, aortic endothelial cells from random bred wild-type strain (WILD) and SUS and atherosclerosis-resistant (RES) Japanese quail were isolated and characterized. GSH levels were higher in the SUS cells and lowest in RES cells, and glutathione reductase was higher in WILD cells than SUS and SUS cells. Subconfluent monolayers of RES cells had higher HO activity compared with SUS cells whereas HO activity levels were similar between strains at confluence. Catalytic iron levels were higher in SUS cells than WILD and RES cells. In the final study of this thesis, aortic endothelial cells were exposed to in-vitro oxidative challenge to further investigate the role of HO and glutathione in the RES and SUS strains. (Abstract shortened by UMI.)