In vivo study of pH Dependent and Enzymatically Triggered Colon Targeted Tinidazole Microspheres

摘要

Introduction: Shellac coated Tinidazole (TNZ) loaded pectin microspheres with the potential for colon targeted deliveryhas been evaluated to study in-vivo behavior. Materials and Methods: Biodistribution and pharmacokinetic studies wereperformed for the determination of concentration of TNZ in rats. This method was used to evaluate the colon targetingproperty of TNZ loaded microspheres. Pure TNZ or TNZ microspheres were given to rats by oral administration. Plasmaand the different parts of gastrointestinal (GI) tract were taken after 2, 8, 12 and 24 h of oral administration of pure TNZor TNZ microspheres to rats and the concentration of TNZ was measured. Results and Conclusion: Results obtainedshows that pure TNZ distributes mainly in stomach and in low concentration to small intestine and colon. However, TNZreleased from microspheres mainly distributes in colon. Therefore, this approach suggests that shellac coated TNZ loadedpectin microspheres has a good colon targeting property.