BACKGROUND: Previous studies have shown that glial cell line-derived neurotrophic factor (GDNF) exhibits good effect on the regeneration of the injured spinal cord.rnOBJECTIVE: To investigate the effect of the combination of the injured chondroitin sulfate ABC (ChABC), GDNF and NogoA microspheres on the regeneration and functional recovery of the injured spinal cord in rats.rnMETHODS: Totally 64 adult SD female rats were randomly divided into eight groups: normal control group, sham operation group (only laminectomy), model group, GDNF group, ChABC microspheres group, GDNF microspheres group, NogoA microspheres group, ChABC+GDNF+NogoA microspheres group. T10 total transected spinal cord models were established in all groups except for normal control and sham operation groups, and the broken ends of the injured spinal cord were injected a half of liquid medicine, and then the back of the broken ends were covered by the other half of liquid medicine which was absorbed by latin sponge.rnRESULTS AND CONCLUSION: 〣asso, Beattie and Bresnahan (BBB) Locomotor Rating scores: ChABC+GDNF +NogoA microspheres group was higher than the model, GDNF, GDNF microspheres, ChABC and NogoA microspheres groups at weeks 5, 6, 7, 8, 9 and 10 after treatment (P < 0.05). 〤ortical somatosensory evoked potential (CSEP) at week 10 after treatment: no detection was found in the sham operation , model and GDNF groups. CSEP in main reaction latency of the ChABC+GDNF+NogoA microspheres group was lower than that of other groups (P< 0.05), but higher than that of the normal control group (P< 0.05). The peak value of the main reaction peak in the ChABC+GDNF+NogoA microspheres group was higher than that in other groups (P < 0.05), but lower than that in the normal control group (P < 0.05). These findings suggest that the combination of GDNF, NogoA and ChABC microspheres can promote the regeneration, functional recovery and nerve conduction of the spinal cord in rats, which is superior to that of pure GDNF microspheres.%背景:前期研究证实胶质细胞源性神经营养因子促损伤脊髓再生修复效果良好.目的:观察胶质细胞源性神经营养因子、NogoA 与硫酸软骨素ABC 缓释微球联合促进大鼠损伤脊髓再生运动功能修复的作用.方法:取64 只SD大鼠随机分为正常对照组、假手术组(仅行椎板切除)、模型组、胶质细胞源性神经营养因子组、胶质细胞源性神经营养因子微球组、硫酸软骨素ABC微球组、NogoA抗体微球组、联合组(胶质细胞源性神经营养因子微球+硫酸软骨素ABC微球+NogoA抗体微球).后6 组制备T10脊髓完全横断伤模型,于脊髓断端间缓慢注射一半相应药液,另用一块明胶海绵吸附另一半相应药液覆盖断端背面.结果与结论:①BBB 运动功能评分:术后5,6,7,8,9,10 周,联合组评分高于模型组、胶质细胞源性神经营养因子组、胶质细胞源性神经营养因子微球组、硫酸软骨素ABC 微球组、NogoA 抗体微球组(P < 0.05).②术后10 周皮质体感诱发电位:假手术、模型组、胶质细胞源性神经营养因子组均未检测出.联合组主反应潜伏期低于其他各组(P < 0.05),但高于正常对照组(P < 0.05);主反应峰峰值高于其他各组(P < 0.05),但低于正常对照组(P <0.05).说明胶质细胞源性神经营养因子缓释微球及NogoA、硫酸软骨素ABC 缓释微球联合促大鼠损伤脊髓再生神经功能修复效果优于单用胶质细胞源性神经营养因子缓释微球.
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