Beyond culture: What is matrix-assisted laser desorption ionization time of flight mass spectrometry?

摘要

Pathogen identification remains a challenge and culture methods have limitations. Novel molecular methods have become clinically available. Matrix-assisted laser desorption ionization-time of flight mass spectrometry — MALDI-TOF — is a new technology for rapid and cost-effective identification of microorganisms, including those implicated in periprosthetic joint infection. Current methods for identification of pathogens rely upon costly and time-consuming culture techniques. MALDI-TOF exploits the proteome of microorganisms for pathogen recognition. It is efficient for implementation at the large institution-level and can precisely classify microorganisms down to the strain.