Optimization of Total Flavonoids Extraction from Coreopsis tinctoria Nutt. by Response Surface Methodology

摘要

Response surface methodology (RSM) was applied to predict optimum conditions for extractionof flavonoid from Coreopsis tinctoria Nutt. A central composite design (CCD) was used to monitorthe effect of extraction temperature, extraction time, and water-to-material ratio on yield of totalflavonoids. The optimal extraction conditions were obtained as water-to-material ratio of 55 mlg?1, extraction temperature of 80 °C and extraction time of 70 minutes. Under these conditions,the average total flavonoids yield, according to the mass of raw material, was 9.0 ± 0.6 %, whichcorresponds to the predicted value of 8.9 %. Thus, the extraction method was applied successfullyto extract total flavonoids from C. tinctoria.