Genotoxicity in Cell Lines Induced by Chronic Exposure to Water-Soluble Fullerenes Using Micronucleus Test

摘要

Objectives: Nanomaterials have numerous potential benefits for society, but the effects of nanomaterials on human health are poorly understood. In this study, we aim to determine the genotoxic effects of chronic exposure to nanomaterials in various cell lines. Methods: Chinese hamster ovary (CHO) cells, human epidermoid-like carcinoma (Hela) cells and human embryonic kidney 293 (HEK293) cells were treated with the water-soluble fullerene C60(OH)24 for 33–80 days. Cell proliferation, cytotoxic analysis and micronucleus tests were performed. Results: When treated with C60(OH)24 (0, 10, 100, or 1000 pg/ml) for 33 days, both the HEK293 and Hela cells showed increased cell proliferation, but cellular lactate dehydrogenase (LDH) activity was not affected. After long-term exposure (80 days) to C60(OH)24 (0, 10, 100, or 1000 pg/ml), the CHO, Hela and HEK293 cells showed increased genotoxicity on the micronucleus test. Conclusion: This study suggests that nanomaterials, such as C60(OH)24, have genotoxic effects.