Extensive studies on polymerase chain reaction-sequence-specific primers (PCR-SSP) based HLA-DRB1* allele profiling in non insulin dependent diabetes mellitus (Indian population)

摘要

Human leukocyte antigen?(HLA) system, the most polymorphic and comprehensively studied gene locus, is extensively used to understand the evolution of genetic relatedness and migration of different world populations. To analyze the contribution of HLA class II genes in type 2 diabetes mellitus, we examined the distribution of HLA- DRB1* loci in Indian population of Tamil Nadu using?polymerase chain reaction-sequence-specific primers?(PCR-SSP). The results were compared with random controls (n=25) of the different area. The present study deals with the genetic association of HLA – DRB1* allele in type 2 diabetic patients. The HLA – DRB1* 15 and HLA – DRB1* 03 were found at higher frequencies than in many other alleles. In controls, DRB1*07 was shown to be at a higher frequency. However, some of the common alleles in the population, such as HLA – DRB1*19 11 were not observed in the present study of diabetic patients. HLA – DRB1*16, 08 and 04 were recorded with less frequency in diabetic patients. In India, type 2 diabetes is significant association (p<0.005) with allele frequency.