Molecular detection of invA and spv virulence genes in Salmonella enteritidis isolated from human and animals in Iran

摘要

It is important to study the genotypic diversity of?Salmonella?plasmid genes which are responsible for its virulence.?In the present study multiplex polymerase chain reaction (multiplex PCR) assay was carried out for detection of?Salmonella enteritidisand presence of?invA?and?spv?genes. In the first stage of the study, 1001 poultry samples were collected from a slaughterhouse in Kerman province (southern Iran). Biochemical and serological tests were then performed for identification ofSalmonella?serovars and 6.79%?(68/1001) were positive for?Salmonella.?Multiplex PCR with?three set primers?was then applied to confirm?serovar?enteritidis 51.4% (35/68).?Simple-PCR was then applied to detect?spvA?(Salmonella?plasmid virulence), and?spvB?genes. Finally, multiplex PCR assay was carried out to simultaneously detect and identify?invA?and?spvC?genes. The presence of?spvA,spvB?and?spvC?in?S. enteritidis?was 88.6% for each gene. In the second stage of the study, thirty-three bovine (n = 13) and human (n = 20)?S. enteritidis?strains were isolated from the culture collection in the Department of Microbiology, Faculty of Veterinary Medicine, University of Tehran, Iran. The analyses of the samples revealed that?spvA, spvB,?and?spvC?genes were present in 90% of?S. enteritidis?from human sources as compared to 100% in bovine sources. The study represents the first report in Iran about the genotypic diversity of?spvA,?spvB?and?spvC?genes of?S. enteritidis.