Screening of higher yield of ethyl acetate yeast and researching on ester-producing conditions

摘要

A higher yield of ethyl acetate yeast Y1 was selected from Daqu and Xiaoqu, and the production volume of ethyl acetate was 2.152 g/l when cultured in corn saccharification mash. The strain is identified as?Hansenula anomala. Then,?through?the single factor test to study the effects of diffident culture mediums and diffident culture methods on?ester-producing, the results showed that the best?ester-producing?culture medium for Y1 yeast was the wheat saccharification mash. The ester yield of static culture was 40% higher when compared with shaking culture. several factors were selected to carry on the orthogonal test once more, the result indicated that the optimization plan were as follows: brix was 8°, pH was 5, temperature was 25°C, culture time was 4 days, and ethyl alcohol was 2%, total ester production of Y1 was 4.812 g/l in this condition.