Analysis of cellular behavior and cytoskeletal dynamics reveal a constriction mechanism driving optic cup morphogenesis

摘要

Tissues and organs form into their final shapes because the cells in a developing embryo generate forces that alter their shape and position. Networks of fibres made from actin and myosin proteins generate these forces, and because the fibres can assemble in many different ways inside cells, they allow the cells to move and change shape in many different ways. Forces in some tissues can cause flat sheets of cells to bend. These sheets of cells are attached on one side (their “basal” surface) to a collection of membranes and molecules that are known as the extracellular matrix. When the cells in the sheet progressively shrink at their basal surface, causing the sheet to bend towards the extracellular matrix, this is known as basal constriction. Nicolás-Pérez et al. have used high-resolution imaging to record how basal constriction helps the optic cup – the main chamber of the eye – to form in zebrafish embryos. This imaging confirmed that a sheet of precursor cells progressively bends towards its basal surface to form the curved shape of the eyeball. Further analysis revealed that this basal constriction happens when myosin fibres accumulate in clusters along the basal surface of some of the precursor cells. The resulting contraction of the basal surface of the cells relies both on the tension generated by myosin inside the cell and on the cells being attached properly to the extracellular matrix. Using a laser beam, Nicolás-Pérez et al. also destroyed small parts of the basal surface of the retina. This procedure allows the mechanical tension distribution throughout the developing eye to be mapped. Laser ablations revealed a narrow time window during development when destroying small parts of the basal surface can cause the entire sheet of cells to relax, preventing it from curving to form the shape of the eye. Sheets of precursor cells are important building blocks of the nervous system, yet researchers only have limited knowledge of the processes that enable them to fold or bend into a final shape. As such, the findings of Nicolás-Pérez et al. will contribute to a wider understanding of how cells and tissues behave while the brain is forming.