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FT-IR analysis and correlation studies on the antioxidant activity, total phenolics and total flavonoids of Indian commercial teas (Camellia sinensis L.) - A novel approach

机译:FT-IR分析和相关研究印度商品茶(Camellia sinensis L.)的抗氧化活性,总酚和总黄酮-一种新方法

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Fourier Transform Infrared (FT-IR) spectra were recorded to analyse the phytochemicals in six commercial green tea (Camellia sinensis L.) samples acquired from diverse locations in India. Main focus was made on the absorbance (%) of selected prominent vibrational bands relevant to the chemical compounds with antioxidant properties (polyphenols, flavonoids). The colorimetric data of the green tea samples such as antioxidant activity assayed by 1,1-diphenylpicrylhydrazyl (DPPH) - radical scavenging activity (RSA) and ferric reducing antioxidant power (FRAP) assay and total phenolic content (TPC) assayed by Folin-ciocalteu method and total flavonoids (TF) assayed by aluminium chloride method were used in the present study (Authors- work communicated elsewhere). A novel attempt has been made to correlate the FT-IR data and the colorimetric data of the green tea samples without using advanced chemometric procedures. Very interestingly excellent correlations were observed among the studied parameters with higher values of correlation coefficients (R2 > 0.9). This implies that FT-IR analysis may be used in a simple way to rapidly estimate antioxidant potentials of plant extracts.
机译:记录了傅里叶变换红外(FT-IR)光谱,以分析从印度不同地点采集的六种商用绿茶(Camellia sinensis L.)样品中的植物化学成分。主要关注与具有抗氧化特性的化合物(多酚,类黄酮)相关的选定突出振动带的吸光度(%)。绿茶样品的比色数据,例如,通过1,1-二苯基吡啶甲基肼基(DPPH)测定的抗氧化活性-自由基清除活性(RSA)和还原铁的抗氧化能力(FRAP)测定以及Folin-ciocalteu测定的总酚含量本研究使用氯化铝法测定的总黄酮含量(TF)和总氯化物(TF)含量(作者在其他地方进行交流)。已经进行了新颖的尝试,以在不使用高级化学计量程序的情况下将绿茶样品的FT-IR数据与比色数据相关联。有趣的是,在相关参数较高的参数之间观察到极好的相关性(R2> 0.9)。这暗示着可以以简单的方式使用FT-IR分析来快速估计植物提取物的抗氧化潜力。

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