Bioprospection of Bioactive Compounds from Spirulina platensis and in-vitro Therapeutic Applications

摘要

The objective of the present research work was to evaluate the phytochemical analysis, antioxidant, anti-inflammatory, antidiabetic,antiurolithiasis, and antimicrobial activities of different extracts obtained from Spirulina platensis. The Spirulina platensis wascollected from Rankala Lake, Kolhapur, India and full-grown in CHU-10 media for 15 days. After 15 days the culture was recovered,dried, powdered and extracted using aqueous, methanol, chloroform, and ethyl acetate by solvent- solvent extraction method.Qualitative and quantitative phytochemical analysis was performed. Further extracts were submerged for different therapeuticapplications. Potent crude extracts were purified by thin layer chromatography and Fourier Transfer Infra Red spectroscopy. Thequalitative phytochemical analysis of all extracts revealed the presence of alkaloids, phenols, flavonoids, tannins, anthroquinones,glycosides, Lignins, sterols, and volatile oils while quantitative analysis revealed 27.0090±0.04129mg of highest phenolic content inthe aqueous extract and 63.47± 0.88059mg of highest flavonoids content in the chloroform extract. Highest ferric ion reducingpower was showed by the aqueous extract at the rate of 1.2407±0.00702 and chloroform extract showed better antioxidant capacityat the rate of 0.8983±0.00351 in Phosphomolybdenum assay. The methanol extract showed highest anti-inflammatory activity andalpha-amylase inhibition at the rate of 85.3200±1.63000 and 50.1907±0.33250 with IC50 value 99.62μg/ml respectively. Theaqueous crude extract showed highest inhibition zone at the 100% concentration against Escherichia coli (16.3±0.57) and Aspergillusniger (20.5 ± 0.24). The maximum calcium oxalate inhibition shown by aqueous crude extract at the rate of 67.5±1.49 with 100%concentration.