FORMULATION AND IN VITRO CHARACTERIZATION OF ANTICANCER DRUG LOADED SOLID LIPID NANOPARTICLES

摘要

The aim of the study was to prepare Temozolomide loaded solid lipid nanoparticles and evaluating loaded solid lipid nanoparticles. Temozolomide loaded solid lipid nanoparticles were prepared by micro emulsification method using different lipid (stearic acid) concentration keeping drug concentration unchanged, poloxamer 188 and cetyl alcohol as surfactant and co-surfactant. The prepared SLN were characterized for surface morphology by SEM analysis, drug loading and % entrapment efficiency, FTIR, zeta potential and in vitro diffusion studies. The lowest and highest % Entrapment Efficiency was found to be 85.85% and 97.45% for F1 to F5 batches respectively. Release studies were done by using saline phosphate buffer pH 7.4 using dialysis bag diffusion method. Zeta potential was found to be – 20.3 mV and particle size was found to be 119.34. For 8 hrs in vitro drug release found to be 79.16%, 74.45%, 71.42%, 61.36%, and 55.13% respectively. The release data was analysed by different kinetic models and found that the formulations best fit model is peppas and the drug release mechanism was Fickian diffusion (n-value 0.3808).