Characterization of Acid and Pepsin Soluble Collagen from the Skin of Horse Mackerels (Magalaspis cordyla) and Croaker (Otolithes ruber)

摘要

Acid and pepsin-soluble collagen was isolated from the skin of horse mackerel (Magalaspis cordyla ) and croaker (Otolithes ruber ) using 0.5 M acetic acid followed by precipitation with 0.9 M NaCl. The yields of acid soluble collagen were 17.3 ± 0.4 and 21.9 ± 0.6% and pepsin soluble collagen (22.5 ± 0.8 and 25.7 ± 0.3%) as per wet weight basis, respectively. The extracted acid soluble collagen and pepsin soluble collagen were characterized by SDS-PAGE, amino acid composition and FTIR spectroscopy. SDS-PAGE showed that all the extracted collagen contained two alpha components (α 1 and α 2) and one beta component (β); all the collagens were typical type I and maintained their triple helical structures well with slight molecular structure differences. The amino acid profiles of these collagens were similar with a low imino acid content. Therefore, there is a possibility to use horse mackerel and croaker skin collagen as an alternative source for commercial collagen and may find applications in the food, cosmetic, biomedical, and pharmaceutical industries.