Evaluation of Antifungal Mechanism of Bacillus amyloliquefaciens BA-16-8

摘要

Penicillium expansum, the pathogen in apple blue moLd, is the primary cause of postharvest apple decay. Fengycin produced by Bacillus amyloliquefaciens BA-16-8 can inhibit the growth of P. expansum. In this study, we explored the antifungal mechanism of fengycin against P. expansum. The effects of fengycin on the morphological structure of P. expansum mycelium were studied via scanning electron microscopy and transmission electron microscopy. The effect of fengycin on the DNA of P. expansum was investigated by conducting electrophoretic mobility shift assay (EMSA). Results showed that after fengycin treatment, the surface of P. expansum became rough and the intracellular protoplasm, particularly the mitochondrion, was damaged by fengycin. EMSA results showed that fengycin can bind nonspecifically with the DNA of P. expansum in vitro. Hence, fengycin can inhibit P. expansum mycelium by altering its cell membrane integrity and binding with its DNA.