首页> 外文期刊>International Journal of Clinical and Experimental Pathology >Podoplanin (D2-40): A New Immunohistochemical Marker for Reactive Follicular Dendritic Cells and Follicular Dendritic Cell Sarcomas
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Podoplanin (D2-40): A New Immunohistochemical Marker for Reactive Follicular Dendritic Cells and Follicular Dendritic Cell Sarcomas

机译:Podoplanin(D2-40):反应性滤泡树突状细胞和滤泡树突状细胞肉瘤的一种新的免疫组化标记

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The diagnosis of follicular dendritic cell (FDC) sarcoma can be challenging because of its morphologic overlaps with many other spindle cell neoplasms and, therefore, new phenotypic markers will be helpful in its differential diagnosis. Podoplanin is a mucin-type transmembrane glycoprotein that has recently been detected in reactive FDCs. In this study, we investigated the expression patterns of podoplanin using a new mouse monoclonal antibody D2-40, and compared them with CD21, a well-established FDC marker, in a comprehensive panel of cases. The panel included 4 FDC sarcomas, 38 spindle cell neoplasms of other types, 25 reactive lymphoid hyperplasia, and 117 lymphoid and 5 myeloid malignant hematopoietic neoplasms. Our study revealed that D2-40 strongly stained 3 of 4 FDC sarcomas. In contrast, D2-40 stained only 2/38 other spindle cell neoplasms tested. Furthermore, we observed that D2-40 highlighted more FDC meshworks than CD21 in Castleman's disease, follicular lymphoma, nodular lymphocyte predominance Hodgkin lymphoma, and residual reactive germinal centers in a variety of lymphoma types. D2-40 and CD21 stained an equal number of cases of reactive lymphoid hyperplasia, progressively transformed germinal centers and angioimmunoblastic T-cell lymphoma. No expression of podoplanin was detected in normal or neoplastic lymphoid and myeloid cells. We conclude that podoplanin (D2-40) is a sensitive and specific FDC marker, which is superior or equal to CD21 in evaluating both reactive and neoplastic FDCs. In addition, our results suggest that podoplanin (D2-40) can be used to support the diagnosis of FDC sarcoma.
机译:滤泡树突状细胞(FDC)肉瘤的诊断可能具有挑战性,因为它与许多其他纺锤状细胞瘤的形态重叠,因此,新的表型标记物将有助于其鉴别诊断。 Podoplanin是一种粘蛋白型跨膜糖蛋白,最近已在反应性FDC中检测到。在这项研究中,我们调查了使用新型小鼠单克隆抗体D2-40的Podoplanin的表达模式,并将其与CD21(一种完善的FDC标记)进行了比较。专家组包括4名FDC肉瘤,38种其他类型的梭形细胞肿瘤,25种反应性淋巴样增生以及117种淋巴样和5种髓样恶性造血肿瘤。我们的研究表明,D2-40对4个FDC肉瘤中的3个强烈染色。相反,D2-40仅染色了2/38所测试的其他梭形细胞肿瘤。此外,我们观察到在各种类型的淋巴瘤中,Castleman病,滤泡性淋巴瘤,结节性淋巴细胞优势霍奇金淋巴瘤和残留的反应性生发中心中,D2-40突出显示的FDC网格比CD21多。 D2-40和CD21对等量的反应性淋巴样增生,逐渐转变的生发中心和血管免疫母细胞T细胞淋巴瘤染色。在正常或赘生性淋巴样和髓样细胞中未检测到podoplanin的表达。我们得出的结论是,podoplanin(D2-40)是一种敏感而特异的FDC标记,在评估反应性和肿瘤性FDC方面优于或等于CD21。此外,我们的结果表明,podoplanin(D2-40)可用于支持FDC肉瘤的诊断。

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