In Vitro Studies to Evaluate the Antistaphylococal Aureus Activities of Propolis: A Wonder Honey Bee Product in Comparison with Antibiotics: Ampicillin and Amoxycillin

摘要

Aims: Propolis: A wonder honey bee product is a natural resinoussubstance called Bee Glue, collected by bees from the exudates andsecretions of plants. The aim of the present study was to determinethe antistaphylococal aureus activities of propolis.Methods and Results: The antibacterial activity of propolis wasdetermined by well diffusion and Macro dilution methods usingethanolic, methanolic and water extracts. Different extracts ofPropolis at the concentrations of 15mg/ml, 30mg/ml, 45mg/ml,60mg/ml and 75mg/ml were prepared and 50ul was added to thewells. After growth, zones of inhibition were determined andcompared with antibiotic Ampicillin (10μg) and Amoxycillin (30μg).The ethanolic extract of propolis showed maximum inhibition(14.3mm) as compared to the methanolic (12.6mm) and water extract(10.7mm). The zone of inhibition of the positive control was found tobe maximum (15.9mm) for Ampicillin and (17.8mm) for Amoxycillin.