IN VITRO MODELING OF HEMATOPOIETIC STEM CELL MEDICINAL NICHES: A TOOL FOR STUDYING REGULATION OF HEMATOPOIESE, EVALUATING THE POTENTIAL OF NICHING A HEMATOPOIETIC GRAFT AND TESTING THE PHARMACO-TOXICOLOGY OF MEDICINAL PRODUCTS.

摘要

The present invention relates to a hematopoietic stem cell (HSC) and / or hematopoietic progenitor (PH) culture support comprising a calcium biomaterial, osteoclasts, endothelial cells and mesenchymal stem cells (MSCs) and / or osteoblasts and / or or adipocytes. The present invention also relates to a method of preparing such a culture medium, and a method of in vitro culture of CSH and / or PH. The use of such a culture medium to study the cellular mechanisms involved in hematopoiesis and / or the differentiation of HSCs / PHs, and / or to study the efficacy and / or toxicity of a drug candidate is also described.