In pH 0–1.0 HCl medium, 12-tungstophosphoric acid (TP) could react with vitamin B1 (VB1) to form a 1?:?3 ion-association complex. As a result, new spectra of resonance Rayleigh scattering appeared and their intensities were enhanced greatly. The maximum wavelength was located at 335 nm. The scattering intensities were proportional to the concentration of VB1 in the range of 0.25–1.75 μg mL?1. Therefore, a new method for the determination of VB1 was established. The method had high sensitivity and good selectivity, and the detection limit (3σ) was 0.94 ng mL?1. It was applied to the determination of VB1 in urine samples and VB1 tablets, which showed that the results were consistent with those of the China pharmaceutical method. The recovery was between 97.3%–99.0%, and the relative standard deviation (RSD) was between 1.1%–3.4%. Furthermore, the optimum reaction conditions, the RRS spectra characteristics, the reaction mechanism and the reasons for enhancement of scattering were evaluated...
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机译：在pH为0-1.0 HCl的介质中，12钨磷酸（TP）可能与维生素B1（VB1）反应形成1？：？3离子缔合复合物。结果，出现了共振瑞利散射的新光谱，并且其强度大大增强。最大波长位于335 nm。散射强度与VB1的浓度成正比，范围为0.25–1.75μgmL？1。因此，建立了测定VB1的新方法。该方法灵敏度高，选择性好，检出限（3σ）为0.94 ng mL？1。用于尿样和VB1片剂中的VB1测定，结果与中国制药法一致。回收率在97.3％至99.0％之间，相对标准偏差（RSD）在1.1％至3.4％之间。此外，还评估了最佳反应条件，RRS光谱特征，反应机理以及增强散射的原因...
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