Effects of Several Antioxidants on Browning Inhibition in in vitro Culture of Leaves Harvested from Adult Plants in Loquat (Eriobotrya japonica L.)

摘要

The attempt in this study was to find efficient methods of overcoming explant browning in leaf culture in which the leaf explants were harvested from adult plants in loquat (Eriobotrya japonica L.). Antioxidants and adsorbents including Na2S2O3, Polyvinyl Pyrrolidone (PVP), AgNO3, Activated Charcoal (AC), citric acid, Vitamin c (Vc) and bleaching powder were employed to study their effects on browning control by immersing explants and adding to the medium. The results indicated that 0.5 mg L-1 AgNO3 added to the medium showed the best effect on browning control while all the other measures did not have obvious effects.