ANTIOXIDATIVE PROPERTIES OF Plantago lanceolata L. EXTRACTS EVALUATED BY CHEMILUMINESCENCE METHOD

摘要

Plantago lanceolata L. is used as astringent, anti-inflammatory, immunoregulatory, antispasmodic, expectorant, emollient,topical anodyne, antibacterial for cuts, bruises and abscesses. These pharmacological properties are due to itscomplex chemical composition: iridoid glycosides, polysaccharides (plantaglucide, glucomannon), terpenoids, coumarins,lignans, caffeic acid derivatives, polysaccharides, flavonoids, flavone glycosides and other constituents, like allantoin.In this paper, antioxidant activity, qualitative and quantitative determination of some active components (flavonoids,polyphenols, and polyphenols carboxylic acids) are evaluated, taking into account the biological properties.Antioxidant properties were evaluated by chemiluminescence (CL) technique in system generator based at 5-amino-2.3-dihydrophthalazine-1.4-dione and hydrogen peroxide, in alkaline medium and DPPH (2.2-diphenyl-1-picrylhydrazyl)method. The total flavonoids, polyphenols content and polyphenol carboxylic acids was spectrophotometrically determinedaccording to Romanian Pharmacopoeia (FR). In addition, the qualitative biochemical fingerprint was investigatedby mass spectrometry LC-ESI-MS. The mass spectral data and amount of active components (flavonoids, polyphenols,polyphenols carboxylic acids) demonstrated the chemical structural complexity. The results of the chemiluminescencein vitro tests revealed that the Plantago lanceolata L. extracts exhibit high antioxidant activity (84-96%), beingin accordance with the value provided by DPPH free radical scavenging assay. Also is established a dependence directlyproportional to the content of polyphenolic compounds found in herbal extracts with level of antioxidant activity.