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首页> 外文期刊>African Journal of Biotechnology >Detection of the introgression loci in Triticum aestivum transferred from Aegilops tauschii by simple sequence repeat (SSR) markers
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Detection of the introgression loci in Triticum aestivum transferred from Aegilops tauschii by simple sequence repeat (SSR) markers

机译:用简单序列重复(SSR)标记检测从牛肝菌转移的普通小麦的基因座

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Aegilops tauschii?possesses many valuable traits or genes. The introgression loci transferred from?A. tauschii?in populations of 64 lines of BC1F2?and 147 advanced lines of BC1F2-F7?derived from the cross of Chinese Spring (CS;?Triticum aestivum) /SQ-214 (A. tauschii)?//SW3243 (T. aestivum) was characterized using simple sequence repeat (SSR) markers. 68 polymorphic loci covering the D genome were used to analyze the distribution of?A. tauschii?SQ-214 introgression loci in the two derived populations. The result shows that 82.35% loci of?A. tauschii?SQ-214 were detected in BC1F2?population,?while the frequency of introgression loci was 51.47% in the advanced lines of BC1F2-F7?after artificial selection?for stripe rust resistance and agronomic traits. Significant changes of the distribution frequency for the same introgression loci between BC1F2?and BC1F2-F7?populations were observed, and seven selective advantage loci from?A. tauschii?(Xgdm128-3D, Xgdm8-3D, Xgdm72-3D, Xgwm341-3D, Xgdm63-5D, Xgdm132-6D and Xgdm36-6D) were found, which introgression loci distribution frequency increased from 29.69 to 53.91% (BC1F2) to 94.22 to 100% (BC1F2-F7). The selective advantage loci could be used to evaluate the desirable genes or traits derived from?A. tauschii?SQ-214.
机译:牛肝菌具有许多有价值的性状或基因。渗入基因座从?A转移。红豆杉中的64个系BC1F2?和147个高级BC1F2-F7?群体来自中国春(CS; Triticum aestivum)/ SQ-214(A。tauschii)?// SW3243(T。aestivum)使用简单序列重复(SSR)标记进行特征分析。用68个覆盖D基因组的多态性位点分析ΔA的分布。 tauschii?SQ-214渗入基因座在两个衍生种群中。结果表明,ΔA的基因座为82.35%。人工选择后,在条带锈病抗性和农艺性状上经过人工选择,在BC1F2的群体中检出了tauschii?SQ-214,而在BC1F2-F7的先进株系中,基因座的渗入频率为51.47%。观察到BC1F2′和BC1F2-F7′种群的相同渗入基因座的分布频率有显着变化,而ΔA有七个选择优势基因座。发现了tauschii?(Xgdm128-3D,Xgdm8-3D,Xgdm72-3D,Xgwm341-3D,Xgdm63-5D,Xgdm132-6D和Xgdm36-6D),其渗入基因座分布频率从29.69%增加至53.91%(BC1F2)至94.22。到100%(BC1F2-F7)。选择性优势基因座可用于评估源自ΔA的理想基因或性状。 tauschii?SQ-214。

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