Suppression of TRPM 7 Inhibits Proliferation, Migration, and Invasion of Malignant Human Glioma Cells

摘要

Summary Background Glioblastoma multiforme ( GBM ) is the most common and aggressive primary brain tumor with a dismal prognosis. Despite intensive study on tumor biology, the underlying mechanisms of the unlimited proliferation and progressive local invasion are still poorly understood, and no effective treatment has been developed for GBM patients. Aims We determine the role of TRPM 7 channels in the growth, migration, and infiltration of malignant glioma cells. Methods Using a combination of RT ‐ PCR , Western blot, and patch‐clamp techniques, we demonstrated the expression of functional TRPM 7 channels of A172 cells, a human glioma cell line, as well as in human glioma tissues. Furthermore, we evaluated the role of TRPM 7 in growth, migration, and infiltration of A172 cells with MTT and transwell migration and invasion assays. Results We showed the expression of functional TRPM 7 channels in both A172 cells and human glioma tissues. Suppression of TRPM 7 expression with TRPM 7‐si RNA dramatically reduced the proliferation, migration, and invasion of A172 cells. Pharmacological inhibition of TRPM 7 channel with 2‐aminoethoxydiphenyl borate (2‐ APB ) showed a similar effect as TRPM 7‐si RNA . Conclusion We demonstrate that human glioma cells express functional TRPM 7 channel and that activation of this channel plays an important role in the proliferation, migration, and invasion of malignant glioma cells. TRPM 7 channel may represent a novel and promising target for therapeutic intervention of malignant glioma.