Detection and cloning of LINE-1 elements in CHO cells

摘要

Long interspersed elements (LINEs, L1s) are non-long terminal repeat (LTR) retrotransposons found in mammalian genomes and account up to 20% of genomic DNA. It has been shown that active L1 elements can cause mutation resulting in disease, genetic variation and polymorphisms and their inactive copies seem to be involved in recombination and rearrangement. L1–encoded products have been detected in a number of tissues including mammalian germ cell tumours, breast carcinomas and a large variety of transformed mouse and human cell lines.