methods of producing a library of eukaryotic cell clones, method of producing a diverse repertoire of ligands, screening method for a cell of a desired phenotype, screening method for a target-recognizing ligand, library, in vitro library of eukaryotic cell clones, eukaryotic cell-containing container, use of a site-specific nuclease, library uses, method for converting single chain antibody (scfv) populations to immunoglobulin (ig) or fragment, and method for reformatting of any two dna elements attached to a vector

摘要

The present invention relates to methods of producing eukaryotic cell libraries encoding a repertoire of binding molecules ("ligands"), wherein the methods use a site-specific nuclease for targeted cell DNA cleavage to enhance integration. specific site of ligand genes through endogenous cell repair mechanisms. eukaryotic cell populations are produced in which a repertoire of ligand-encoding genes are integrated into the desired locus in the cell DNA (e.g., a genomic locus) allowing expression of the encoded binding molecule thereby creating a population of cells expressing the different ligands.