method for immortalizing a cell population, cell population, oligoclonal or monoclonal cell population, cell culture, cell culture supernatant, DNA library, use of a cell population, cell culture, DNA supernatant or library, kit to identify and produce a monoclonal antibody, method to produce a cell culture, and method to produce a monoclonal antibody

摘要

METHOD FOR IMMORTALIZING A CELL POPULATION, CELL POPULATION, OLIGOCLONAL OR MONOCLONAL CELL POPULATION, CELL CULTURE SURVEYING, CELLULAR CUBULATED BIBLIOTE CELLULATION Identify and Produce a Monoclonal Antibody, Method for Producing a Cell Culture, and Method for Producing a Monoclonal Antibody. The present invention provides methods for immortalizing antibody-secreting cells of one or more specific isotypes. Polyclonal, oligoclonal and monoclonal cell populations obtained by use of the methods of the invention may be screened based on the functional and / or binding activities of secreting antibodies, for example directed to human or viral antigens of medical interest. under cell culture conditions. Using these methods, human B cells, which secrete antibodies by binding to cytomegalovirus, herpes simplex virus, or HSP60 protein, were efficiently immortalized with Epstein-Barr virus.