Determination of Central Nervous and Organ Tissue in Meat Products through GC-MS Analysis of Marker Fatty Acids from Sphingolipids and Phospholipids

摘要

C_(22)-C_(26) fatty acids from sphingo- and phospholipids were used for the determination of CNS material (BSE) and organ tissue (declaration) in meat products. The lipids of interest were isolated from the bulk lipids using a silica gel cartridge, the fatty acids converted to methyl esters, silylated, and analyzed by GC-MS. Two fractions of lipids were analyzed separately, as this enhanced the selectivity of the method. No CNS material was detected in about 120 samples. Only in six of some 60 samples non-declared organ tissue was found - all mortadella, which is traditionally prepared with pork stomach (declaration required in Switzerland). The detection limit for stomach is around 3 %. Other organ tissues shown to be detectable by the method are liver, kidney, and spleen.