ASSOCIATION OF PARATHYROID HORMONE GENE POLYMORPHISM WITH BONE MINERAL DENSITY IN CHINESE WOMEN

摘要

Objective. To investigate the distribution frequency of parathyroid hormone(PTH) gene polymorphism in healthy adults from Bejing area and to explore the association of PTH genotypes with bone mineral density (BMD) . Methods. PTH gene polymorphism was detected in 270 subjects by polymerase chain reaction (PCR) and PCR/restriction fragment length polymorphism (PCR/RFLP). The digestion products of restriction enzyme Bst B1 were separated on 1% agarose gels. PTH genotypes were confirmed by DNA sequences analysis. BMD was measured by dual-energy X-ray absorptiometry (DEXA, DPX-L, Lunar). Results. Genotype frequencies of BB, Bb, bb were 73.7%, 25.9% and 0.4% respectively in Beijing adults (P<0.01), 67.1%, 32.2% and 0.7% in postmenopausal women, which was different from Japanese women. We statistically compared bone mineral density at the lumbar 2-4 and proximal femur between BB and Bb genotypes. No obvious association was found between the BMD and PTH genotypes in Beijing women( P>0.05). Conclusion. PTH gene polymorphism is not associated with BMD in Chinese women. The further research to explore the genetic risk factors of osteoporosis should be committed.