甲状旁腺激素基因多态性对广东地区老年人骨密度的影响

摘要

目的:探讨甲状旁腺素(PTH)基因多态性与中国广东地区部分老年人群骨密度的相关性.方法:随机筛选广东老年人549例,年龄65～87岁,平均(71.03±7.37)岁,采用双能X线吸收测定其腰椎侧位、股骨颈、粗隆间、大转子、ward's三角等部位的骨密度值.用聚合酶链反应及限制性片段长度多态性技术检测外周血白细胞基因组PTH基因型.结果:549例受试对象中,PTH基因BB型382例,69.6%;Bb型148例,27.0%;和bb型19例,3.4%.无论是整个受试群体,还是根据性别将其分为男性及女性群体,其等位基因及基因型分布均符合Hardy-Wenbeng平衡定律.分析基因型与骨密度的关系显示,不同的基因型之间的骨密度均无统计学意义变化(P>0.05).结论:PTH基因多态性与广东地区汉族老年人群骨密度关系不密切,不能作为筛查和预示骨质疏松症的遗传易感位点.%Objective To Investigate the correlation between the gene polymorphisms of parathyroid hormone (PTH) and bone mineral density (BMD) in the elderly in Guangdong district. Methods Five hundred and forty-nine subjects were randomly chosen from Guangdong district [aged 65 ～ 87 years , (71.03±7.37) years ].The genotypes of PTH were detected by polymerase chain reaction-restriction fragment length polymorphism (PCR-RELP), and the BMD of lumhar, inter, trach, vertebrae, femur neck, and Ward's were measured by dualenergy X-ray absorptionmetry ( DEXA). Results The distribution of PTH genotypes were : BB 69.6％ (382 cases) ,Bb 27.0％ (148 cases),bb 3.4％(19 cases). The distribution of PTH genotypes was in accordance with HardyWeinberg equilibrium. There was no significant difference in BMD among cases with different PTH genotypes (P ＞0.05). Conclusion There is no relationship between gene polymorphisms of PTH and BMD in the elderly in Guangdong district.