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首页> 外文期刊>Chemical Communications >Using the ubiquitous pH meter combined with a loop mediated isothermal amplification method for facile and sensitive detection of Nosema bombycis genomic DNA PTP1
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Using the ubiquitous pH meter combined with a loop mediated isothermal amplification method for facile and sensitive detection of Nosema bombycis genomic DNA PTP1

机译：使用无处不在的pH计结合回路介导的等温扩增方法，可简便，灵敏地检测Nosema bombycis基因组DNA PTP1

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摘要

Here we show an amplification-coupled detection method for directly measuring released hydrogen ions during the loop mediated isothermal amplification (LAMP) procedure by using a pH meter. The genomic DNA of Nosema bombycis (N. bombycis) was amplified and detected by employing this LAMP-pH meter platform for the first time.
机译：在这里，我们展示了一种放大耦合检测方法，可通过使用pH计直接在回路介导的等温扩增（LAMP）过程中测量释放的氢离子。首次使用该LAMP-pH测量仪平台扩增和检测了Nosema bombycis（N. bombycis）的基因组DNA。

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  • 来源
    《Chemical Communications》 |2014年第100期|15932-15935|共4页
  • 作者

    Shunbi Xie; Yali Yuan; Yue Song; Ying Zhuo; Tian Li; Yaqin Chai; Ruo Yuan;

  • 作者单位

    State Key Laboratory of Silkworm Genome Biology, Key Laboratory of Luminescent and Real-Time Analytical Chemistry, Ministry of Education (Southwest University), College of Chemistry and Chemical Engineering, Southwest University, Chongqing 400715, PR China;

    State Key Laboratory of Silkworm Genome Biology, Key Laboratory of Luminescent and Real-Time Analytical Chemistry, Ministry of Education (Southwest University), College of Chemistry and Chemical Engineering, Southwest University, Chongqing 400715, PR China;

    State Key Laboratory of Silkworm Genome Biology, Key Laboratory of Luminescent and Real-Time Analytical Chemistry, Ministry of Education (Southwest University), College of Chemistry and Chemical Engineering, Southwest University, Chongqing 400715, PR China;

    State Key Laboratory of Silkworm Genome Biology, Key Laboratory of Luminescent and Real-Time Analytical Chemistry, Ministry of Education (Southwest University), College of Chemistry and Chemical Engineering, Southwest University, Chongqing 400715, PR China;

    State Key Laboratory of Silkworm Genome Biology, Key Laboratory of Luminescent and Real-Time Analytical Chemistry, Ministry of Education (Southwest University), College of Chemistry and Chemical Engineering, Southwest University, Chongqing 400715, PR China;

    State Key Laboratory of Silkworm Genome Biology, Key Laboratory of Luminescent and Real-Time Analytical Chemistry, Ministry of Education (Southwest University), College of Chemistry and Chemical Engineering, Southwest University, Chongqing 400715, PR China;

    State Key Laboratory of Silkworm Genome Biology, Key Laboratory of Luminescent and Real-Time Analytical Chemistry, Ministry of Education (Southwest University), College of Chemistry and Chemical Engineering, Southwest University, Chongqing 400715, PR China;

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  • 入库时间 2022-08-17 13:16:48

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