Isolation and Characterization of a Novel Lectin from the Wild Mushroom Oudemansiella radicate: (Relhan.: Fr.) Sing.

摘要

A novel lectin (ORL), with a molecular mass of 30 kDa and a unique amino acid sequence, was purified from dried fruiting bodies of the mushroom Oudemansiella radicata (Relhan.: Fr.) Sing. ORL is a dimer made of two 15-kDa subunits. The purification procedure encompassed ion exchange chromatography on DEAE-cellulose and Q-Sepharose and fast protein liquid chromatography-gel filtration on Superdex 75. The hemagglutinating activity of ORL was stable at temperatures up to 70℃ and between pH 2.0 ～ 11.0, and was reduced at higher temperatures, and under acidic and alkaline conditions. The activity was activated by Cu~(2+), Al~(3+), Fe~(3+), and Pb~(2+) ions, and inhibited by Hg~(2+) and Fe~(2+) ions. ORL showed no specificity toward the carbohydrates tested. Unlike previously reported mushroom lectins, ORL had no inhibitory effect on FflV-1 reverse transcriptase and proliferation of hepatoma HepG2 cells, as well as breast cancer MCF7 cells, when tested up to 500 μM.