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Different assembly of acid and salt tolerance response in two dairy Listeria monocytogenes wild strains

机译:两种单核细胞增生李斯特氏菌野生菌株对酸和盐的耐受性响应的组装不同

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摘要

A lack on the association between acid tolerance response (ATR) and osmotolerance response (OTR) among Listeria monocytogenes dairy isolates was found. In order to evaluate how wild L. monocytogenes isolates mount tolerance responses under a sub-lethal pH and a low sodium chloride concentration (pH 5.5 and 3.5 % [w/v] NaCl), a proteomic approach was used. The ATR and OTR of two L. monocytogenes cheese dairy isolates (strain T8, serotype 4b and A9, serotype 1/2b or 3b) were determined. The proteomes of the adapted and non-adapted cultures were evaluated by 2-DE. One strain displayed an ATR, but not an OTR and the other displayed an OTR, but not an ATR. The ATR positive strain showed the over-production of proteins related with protein synthesis, protein folding, attainment of reduction power, ribose production and cell wall. In contrast, in the OTR-positive-strain proteins related with glycolysis, general stress and detoxification were identified.
机译:发现单核细胞增生李斯特氏菌乳制品分离株之间的耐酸反应(ATR)和渗透压反应(OTR)之间缺乏关联。为了评估野生单核细胞增生李斯特菌如何在亚致死pH和低氯化钠浓度(pH 5.5和3.5%[w / v] NaCl)下分离安装耐受反应,使用了蛋白质组学方法。测定了两个单核细胞增生李斯特氏菌干酪乳制品分离株(菌株T8,血清型4b和A9,血清型1 / 2b或3b)的ATR和OTR。通过2-DE评估适应的和非适应的培养物的蛋白质组。一种菌株显示ATR,但不显示OTR,另一种菌株显示OTR,但不显示ATR。 ATR阳性菌株显示与蛋白质合成,蛋白质折叠,获得还原能力,核糖产生和细胞壁有关的蛋白质的过量生产。相反,在与糖酵解相关的OTR阳性菌株蛋白中,鉴定出一般应激和排毒。

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