Thermal Stabilization of Enzymes Immobilized within Carbon Paste Electrodes

摘要

In this note we report on the remarkable thermal stabi- lization of enzymes immobilized in carbon paste elec- trodes, Amperometric biosensors are shown for the first time to withstand a prolonged high-temperature(＞50deg) stress. Nearly full activity of glucose oxidase is retained over periods of up to 4 months of thermal stress at 60- 80deg. Dramatic improvements in the thermostability are observed for polyphenol oxidase, lactate oxidase, alcohol oxidase, horseradish peroxidase, and amino acid oxidase.