TGF-β1 secreted by pancreatic stellate cells promotes stemness and tumourigenicity in pancreatic cancer cells through L1CAM downregulation

摘要

Boxplots showing the differential expression of in PDAC samples versus normal tissue (NP) in the indicated series of transcriptomic data. *  p b Immunohistochemistry for L1CAM (brown) in tissue sections from normal pancreas (P) and patients with PDAC at G1, G2 and G3 grade. -score for L1CAM expression. qPCR analysis of and CSCs genes in adherent cells versus spheres. Data are normalised to GAPDH expression and are presented as fold change (FC) in gene expression relative to adherent cells. *  p p n ≥ 6. Western blot analysis for L1 in adherent cells versus spheres. Parallel β-ACTIN immunoblotting was performed and signal quantification was calculated by densitometric analysis. Flow cytometry quantification for L1 in adherent cells compared to spheres. All cytometry gates were established based on isotype controls. *  p p n ≥ 4. Representative immunofluorescence images for L1 (red) and nuclei (blue, DAPI) of adherent cells and spheres. Representative flow cytometry for L1 in subcutaneous tumours derived from L3.6pl injected cells treated with vehicle (H O) or gemcitabine. All cytometry gates were established based on isotype controls.  ≥ 4. Flow cytometry quantification for L1 and CD133 in subcutaneous tumours derived from L3.6pl injected cells treated with vehicle (H O) or gemcitabine.