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Amino Acid Composition of Novel Plant Drinks from Oat Lentil and Pea

机译:燕麦扁豆和豌豆中新颖植物饮料的氨基酸组成

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摘要

Plant-based drinks (PBDs) as alternatives to milk is a fast-growing market in much of the western world, with the demand increasing every year. However, most PBDs from a single plant ingredient do not have an amino acid profile that matches human needs. Therefore, this study set out to combine plant ingredients to achieve a more balanced amino acid profile of novel plant drinks, by combining a high content of oat with the pulses pea ( ) and lentil ( ) in a solution. After removal of the sediment, the resulting plant drinks were composed of what could be kept in suspension. The amino acid and protein composition of the plant drinks were investigated with capillary electrophoresis, to identify the amino acids, and SDS-PAGE to assess the proteins present. The amino acid profile was compared against recommended daily intake (RDI). It was determined that the plant drinks with only oat and lentil did not have a strong amino acid profile, likely due to the higher pH of the lentil concentrate affecting which proteins could be kept in solution. Plant drinks with a combination of both lentil and pea, or only pea, added to the oat drink had an improved concentration of the amino acids that were otherwise in the low end compared to RDI. This includes a high content of phenylalanine, leucine and threonine, as well as a moderate amount of isoleucine, valine and methionine, and a contribution of histidine and lysine. An assessment of stability and sensory parameters was also conducted, concluding there was an advantage of combining oat with a legume, especially pea.
机译:在西方世界的大部分地区,植物性饮料(PBDs)替代牛奶是一个快速增长的市场,需求每年都在增长。但是,大多数来自单一植物成分的PBD都没有符合人类需求的氨基酸谱。因此,这项研究着手通过将高含量的燕麦与豆荚豌豆()和小扁豆()组合在一起,从而结合植物成分,从而实现新型植物饮料的氨基酸平衡。除去沉淀物后,所得植物饮料由可以悬浮的成分组成。用毛细管电泳研究植物饮料的氨基酸和蛋白质组成,以鉴定氨基酸,并通过SDS-PAGE评估存在的蛋白质。将氨基酸谱与建议的每日摄入量(RDI)进行比较。已确定仅含燕麦和小扁豆的植物饮料不具有很强的氨基酸谱,这可能是由于小扁豆浓缩液的较高pH影响了可以将哪些蛋白质保留在溶液中。与RDI相比,燕麦饮料中添加了扁豆和豌豆(或仅豌豆)的植物饮料氨基酸含量得到了改善。这包括高含量的苯丙氨酸,亮氨酸和苏氨酸,以及适量的异亮氨酸,缬氨酸和蛋氨酸,以及组氨酸和赖氨酸。还对稳定性和感官参数进行了评估,得出结论认为燕麦与豆类,尤其是豌豆结合使用具有优势。

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