New Coumarins and Anti-Inflammatory Constituents from the Fruits of Cnidium monnieri

摘要

The fruit of Cnidium monnieri is commercially used as healthcare products for the improvement of impotence and skin diseases. Three new coumarins, 3'-O-methylmurraol (>1), rel-(1'S,2'S)-1'-O-methylphlojodicarpin (>2), and (1'S,2'S)-1'-O-methylvaginol (>3), have been isolated from the fruits of C. monnieri, together with 14 known compounds (>4–>17). The structures of these new compounds were determined through spectroscopic and MS analyses. Compounds >1, >4–>12, and >14–>17 exhibited inhibition (IC50 ≤ 7.31 µg/mL) of superoxide anion generation by human neutrophils in response to formyl-l-methionyl-l-leucyl-l-phenylalanine/cytochalasin B (fMLP/CB). Compounds >7, >9–>11, >15, and >17 inhibited fMLP/CB-induced elastase release with IC50 values ≤7.83 µg/mL. This investigation reveals that bioactive isolates (especially >6, >7, >14, and >17) could be further developed as potential candidates for the treatment or prevention of various inflammatory diseases.